472 Nicotinamide improves cutaneous field cancerization on an ex-vivo study

Cutaneous field cancerization (FC) is a tissue area chronically exposed to sun light, in which cells present altered cell cycle, proliferation, differentiation, and death despite their normal appearance. On sun-exposed skin the most mutated gene p53 regulates expression of p21 controls cycle progression cellular differentiation. Treatment cutaneous FC with safe low-cost molecules could reduce probability cancer recurrence. Nicotinamide (NAM) amide form vitamin B3, water-soluble molecule assumed by daily diet. NAM cheap, used for treatment several diseases. Therefore, this study aimed understand whether improve through ex-vivo experiments. We obtained full-thickness biopsies from 10 healthy patients, as control group, 40 NMSCs diagnosed AK, BCC or SCC. isolated epidermis that was cultured with/without 25 μM 18 24 hours on culture medium incubated at 37°C. Through qRT-PCR we evaluated inflammatory (IL-6, IL-10), differentiation (CK10, CK14) (p53, p21, p16, NOTCH1) markers, while western blotting analyzed CK10, CK14 protein expression. found p53, NOTCH1 lower FC-skin comparison samples. However, slightly increased other hand, p16 more expressed groups. In addition, reduced CK10 FC-skin, trends reverted NAM. Finally, higher level IL-6 IL-10 compared Overall, our results suggest alteration, poor activation inflammation. seemed condition.